|
KMID : 0352720140380030220
|
|
Journal of Ginseng Research
2014 Volume.38 No. 3 p.220 ~ p.225
|
|
|
Plant regeneration of Korean wild ginseng (Panax ginseng Meyer) mutant lines induced by ν-irradiation (60℃) of adventitious roots
|
|
Zhang Jun-Ying
Sun Hyeon-Jin
Song In-Ja
Bae Tae-Woong
Kang Hong-Gyu
Ko Suk-Min
Kwon Yong-Ik
Kim Il-Woung
Lee Jae-chun
Park Shin-Young
Lim Pyung-Ok
Kim Yong-Hwan
Lee Hyo-Yeon
|
|
|
Abstract
|
|
|
|
An efficient in vitro protocol has been established for somatic embryogenesis and plantlet conversion of Korean wild ginseng (Panax ginseng Meyer). Wild-type and mutant adventitious roots derived from the ginseng produced calluses on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2,4-dichlorophenoxyacetic acid and 0.3 mg/L kinetin; 53.3% of the explants formed callus. Embryogenic callus proliferation and somatic embryo induction occurred on MS medium containing 0.5 mg/L 2,4-dichlorophenoxyacetic acid. The induced somatic embryos further developed to maturity on MS medium with 5 mg/L gibberellic acid, and 85% of them germinated. The germinated embryos were developed to shoots and elongated on MS medium with 5 mg/L gibberellic acid. The shoots developed into plants with well-developed taproots on one-third strength Schenk and Hildebrandt basal medium supplemented with 0.25 mg/L 1-naphthaleneacetic acid. When the plants were transferred to soil, about 30% of the regenerated plants developed into normal plants.
|
|
|
KEYWORD
|
|
|
mutant adventitious roots, Panax ginseng Meyer, somatic embryo, somatic embryogenesis
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|